Ire1 knockout

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August undefined, 2024

WebHuman ERN1 (IRE1) knockout HeLa cell line (ab255389) Datasheet. SDS. Submit a review Submit a question. $3,435.00 Product size. 1 × 10 6 cells/vial. Add to basket. Order now and get it on Friday March 03, 2024. Custom quote Bulk request. WebIRE1 has two catalytic domains, an endoribonuclease domain and a kinase domain. The endonuclease activity is responsible for the non-conventional cytosolic splicing of a 26-base intron in the X box binding protein 1 (XBP1) mRNA, leading to a …

Inhibiting the IRE1α Axis of the Unfolded Protein Response …

WebNov 23, 2024 · The role of Ire1 in muscle regeneration was tested in mice with a germline conditional deletion of Ire1 under a muscle creatine kinase promotor. The conditional Ire1 knockout diminished myofiber size out to at least day 14 after injury, reduced numbers of Pax7(+) nuclei in regenerating muscle, and reduced numbers of EdU(+) nuclei. WebSep 27, 2010 · Here, we analyzed the phenotype of IRE1α conditional knockout mice and found that IRE1α-deficient mice exhibit mild hypoinsulinemia, hyperglycemia, and a low … theoretical \\u0026 applied mechanics letters

Development of a Rapid in vivo Assay to Evaluate the Efficacy of IRE1 …

WebSep 12, 2013 · In our opinion, the meaningful way to create a comparison group to the knockout cells is to rescue them with an IRE1 transgene. We had done something similar, … WebMay 26, 2024 · The following antibodies were used: Anti‐WEE1 (1:1000, Abcam#ab233540), Phospho‐CDK1 (Tyr15) (1:500, ABclonal#AP0016), Anti‐γH2AX (phospho‐S140) (1:1000, … WebFeb 5, 2013 · IRE1αis a ubiquitously expressed gene whose deletion results in early embryonic lethality (23). We demonstrated that this lethality was caused by failure in placenta development by generating nonlethal conditional knockout mice that express IRE1α only in the placenta (24). theoretical \\u0026 applied mechanics letters几区

Caspase-mediated cleavage of IRE1 controls apoptotic …

WebER-stressed double-knockout cells showed deficient IRE1-alpha signaling. Bax and Bak formed a protein complex with the cytosolic domain of Ire1-alpha that was essential for Ire1-alpha activation. Thus, Hetz et al. (2006) concluded that BAX and BAK function at the ER membrane to activate IRE1-alpha signaling and to provide a physical link ... WebCRISPR/Cas9 KO Plasmids consists of IRE1α-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library For CRISPR gene knockout, gRNA sequences direct the Cas9 … theoretical \u0026 applied mechanics letters 影响因子WebMay 26, 2024 · Here, it is demonstrated that the WEE1 inhibitor AZD1775 induces endoplasmic reticulum stress and activates the protein kinase RNA-like ER kinase (PERK) … theoretical \\u0026 applied mechanics

"WebPlease refer to our lysis protocol for further details on how our lysates are prepared. User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage. Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. " - Ire1 knockout

Ire1 knockout

Inflammation promotes adipocyte lipolysis via IRE1 kinase

WebJun 22, 2024 · (B) RT-PCR analysis of stress-dependent XBP1 mRNA splicing in WT U-2 OS cells, IRE1 knock-out (KO) U-2 OS cells, and U-2 OS cells in which IRE1 has been fully … WebApr 20, 2024 · In this study, by employing a diverse array of molecular approaches, including biochemical analyses, fluorescence microscopy imaging, and infection assays using primary cells derived from Ern1 (encoding IRE1) conditional knockout mice, we address this gap in our understanding by demonstrating that a novel IRE1α to ULK1, an important component ...

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WebAdiponectin-Cre-mediated IRE1 knockout in mice showed that adipocyte IRE1 was required for inflammatory ligand-induced lipolysis in adipose tissue explants and that adipocyte … WebSanta Cruz Biotechnology, Inc. offers a broad range of IRE1 antibodies. Select IRE1 antibodies from monoclonal antibodies listed below. View detailed IRE1 antibody specifications by linking to the specific product blocks. Select appropriate IRE1 antibodies for your research by isotype, epitope, applications and species reactivity. To the left ...

WebDec 26, 2024 · IRE1α, IRE1β, ATF6α and ATF6β are ubiquitously expressed in medaka. We found that IRE1α/ATF6α-double knockout is lethal, similarly to IRE1α/IRE1β- and ATF6α/ATF6β-double knockout. Therefore, IRE1 inhibitors are expected to confer lethality to ATF6α-knockout medaka but not to wild-type medaka. WebMay 26, 2024 · Conversely, several SASP cytokines induced upon AZD1775 treatment was not reversed but slightly enhanced by IRE1 knockout ( IRE1ko) (Figure 2L; and Figure S3E, Supporting Information), indicating that SASP was not …

WebMay 6, 2024 · IRE1α modulates the expression of genes involved in TAG metabolism To gain further insights into the molecular mechanisms underlying lipid remodeling upon IRE1α … WebAlthough enterocyte-specific Sel1L-knockout mice (Sel1L(ΔIEC)) are viable and seem normal, they are highly susceptible to experimental colitis and inflammation-associated …

Webknockout animals that PERK and ATF6 as well as IRE1 are required for the full activation of the UPR (14, 17–19). PERK, ATF6, and IRE1 have common features as UPR induc-ers. Nevertheless, there are several differences among their func-is essential for the viability of exocrine and endocrine pancreatic cells (20).

WebDouble-knockout mice responded abnormally to tunicamycin-induced ER stress in the liver, with extensive tissue damage and decreased expression of the IRE1 substrate Xbp1 and … theoretical \\u0026 applied mechanics letters 影响因子WebIRE1 is an important central regulator of unfolded protein response (UPR) in the endoplasmic reticulum (ER) because of its ability to regulate cell fate as a function of … theoretical \u0026 applied mechanics letters几区WebActive Recombinant mouse IRE1 protein (Active) is a Baculovirus infected Sf9 Protein fragment 469 to 977 aa range, > 90% purity and validated in FuncS, SDS-PAGE. ... Knockout . Human ERN1 (IRE1) knockout HeLa cell line (ab255389) Primary . Anti-IRE1 (phospho S724) antibody (ab48187) ... theoretical \\u0026 applied scienceWebFeb 16, 2013 · The endoplasmic reticulum (ER) stress response is a highly conserved mechanism that results from the accumulation of unfolded or misfolded proteins in the ER. The response plays an important role in allowing plants to sense and respond to adverse environmental conditions, such as heat stress, salt stress and pathogen infection. Since … theoretical \u0026 applied scienceWebJan 25, 2024 · The requirement of the IRE1 and PERK arms for tumor growth in nude mice is established. Here, we investigated the requirement for the ATF6 arm, which consists of ubiquitously expressed ATF6α and ATF6β, by constructing ATF6α-knockout, ATF6β-knockout and ATF6α/β-double knockout in HCT116 cells derived from human colorectal … theoretical \\u0026 computational scienceWebProduct name Human ERN1 (IRE1) knockout HeLa cell line Parental Cell Line HeLa Organism Human Mutation description Knockout achieved by using CRISPR/Cas9, 1 bp … theoretical \u0026 experimental probabilityWebData (including data from studies in knockout and transgenic mice) suggest that Ep300 and Crebbp are limiting cofactors for pancreatic islet development (including gene expression regulation and cell proliferation), and hence for postnatal glucose homeostasis, with some functional redundancy. ... (ER) stress and P300 activity via the XBP1/IRE1 ... theoretical \\u0026 experimental probability