Phospho irf7
WebJan 20, 2024 · Expression of phospho-IRF7 and nuclear translocation of IRF7 was examined in these extracts by immunoblotting. Pancreatic expression of IRF7 was assessed by immunofluorescence analysis in experimental AIP. Nuclear translocation of IRF7 upon exposure to neutrophil extracellular traps (NETs) was assessed in peripheral blood pDCs … WebIRF7: Gene ID: 3665: Uniprot ID: IRF7_HUMAN: Specificity: Phospho-IRF7-Ser471/472 polyclonal antibody (Interferon Regulatory Factor 7) binds to endogenous Interferon …
Phospho irf7
Did you know?
WebMar 26, 2015 · IRF7 can be activated by RIG-I recognition of IAV genomic RNA, resulting in C-terminal serine phosphorylation by the IKK-related kinases TBK1 and IKK-ε ( 11, 14 – 20 ). Wild-type IRF7 was phosphorylated upon overexpression of TBK1, as was F410V ( Fig. 1D ).
WebOct 7, 2012 · Because commercially available phosphospecific IRF7 antibodies are only sufficiently sensitive to detect overexpressed IRF7, we characterized the poly (I:C)-induced phosphorylation of IRF7... WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH …
WebJan 3, 2014 · An anti-phospho-IRF7 antibody was used for detection of phosphorylated form of IRF7. IRF7 was present predominantly in an unphosphorylated form in cells transfected with IRF7 alone . Co-expression of MyD88, TRAF6 and IKKα enhanced the level of phosphorylated IRF7, whereas this enhancement was strikingly suppressed by co … WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: …
WebSep 1, 2024 · Irf3-/-Irf7-/- MEFs were seeded on 24-well plates (1 ×10 5 cells/well) and transfected the following day with 50 ng of luciferase reporter plasmid together with 0.8 μg of expression plasmid or empty control plasmid via Lipofectamine 2000 (Invitrogen).
WebPhospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb recognizes endogenous levels of IRF-7 protein only when phosphorylated at Ser477. This antibody can also detect IRF-7 when … orbix breast supporting systemWebIRF7 Antibodies Antibodies that detect IRF7 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry and ELISA. These antibodies target IRF7 in Human, Mouse, Rat and Zebrafish samples. ippb access points near meWebJan 8, 2024 · (E, F) Flow cytometry analysis of phospho-IRF7 in CAL-1 WT and zDHHC2 −/− cells after gardiquimod stimulation. (E) Representative histogram of IRF7 phosphorylation level at 60 min after gardiquimod stimulation. (F) MFI of phospho-IRF7 at 0, 60, and 90 min after gardiquimod treatment, compared between WT and zDHHC2 −/− cells (n = 3, mean ... orbix flightsimWebAnti-Phospho-IRF7-S471/472 Antibody from Assay Genie is a high quality antibody with reactivity against Human, Mouse and validated in WB applications. Toggle menu Select Currency: EUR ippb account benefitWeb精脒;精胺N1-乙酰基转移酶1ELISA检测试剂盒相关名称：人淋巴毒素αLTA （Human lymphotoxin Alpha,LTA） ELISA Kit 96T 鱼类状腺原氨酸Fish i-iodothyronine,T3 ELISA Kit 96T ippb account creationWebMar 29, 2024 · The juxtaposition of both the ubiquitination site and phospho-acceptor site on IRF7 and other IRFs suggests that such these post-translational modifications work sequentially to recruit in all the players necessary for activation. And similar to STING, it appears that competing ubiquitin or ubiquitin-like modifications work to fine-tune and ... ippb account statementWebSep 13, 2012 · Baseline levels of phosphorylated IRF7 were found to be similar between males and females. However we observed faster phosphorylation kinetics of IRF7 in females than in males using flow cytometry with phospho-IRF7 peaking in females at 20min post-stimulation and males at 30min. orbix education sdn bhd