Sfp phosphopantetheinyl transferase
Web17 Sep 2013 · We determined the crystal structure of the group II phosphopantetheine transferase Sfp from Bacillus in complex with a substrate carrier protein in the presence … Web25 Sep 2024 · Using the 4′-phosphopantetheinyl transferase (Sfp) enzyme, coenzyme A(CoA)-bearing moieties can be covalently cross-linked to a serine in the ybbR-tag 6,7.
Sfp phosphopantetheinyl transferase
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Web6 Jun 2016 · Null mutation of zmsO blocks the antibiotic activity and decreases the pathogenicity of D. zeae. Bioinformatics and functional analysis showed that ZmsO is a … Web16 Jun 2009 · Function Activates the seven peptidyl carrier protein (PCP) domains of the first three subunits (SrfAA, SrfAB and SrfAC) of surfactin synthetase by transferring the 4' …
Webing Sfp-type phosphopantetheinyl transferase (PPTase), which modifies the “apo” form to “holo” form carrier protein on type I PKSs and NRPSs, were widely distributed in … Web17 Jun 1999 · The Bacillus subtilis Sfp protein activates the peptidyl carrier protein (PCP) domains of surfactin synthetase by transferring the 4'-phosphopantetheinyl moiety of …
WebIn filamentous fungi, Sfp-type 4'-phosphopantetheinyl transferases (PPTases) activate enzymes involved in primary (alpha-aminoadipate reductase [AAR]) and secondary … WebSmall molecules of diverse structures were conjugated to coenzyme A (CoA) and then covalently attached to the phage displayed PCP by Sfp phosphopantetheinyl transferase. …
WebA protocol for site-specific protein labeling by Sfp-catalyzed protein post-translational modification that includes construction of target protein fusions with PCP or the ybbR tag …
Web1 Jan 2024 · Bacillus subtilis surfactin phosphopantetheinyl transferase Sfp produced in E. coli mediated the conjugation of firefly luciferase N-terminally extended by eleven amino … rory mcilroy golfer net worth 2021Web27 Dec 2002 · 4′-phosphopantetheine transferase. Sfp. PPTase involved in surfactin production. HPLC. high pressure liquid chromatography. BD. binding domain. aa. amino … rory mcilroy grip sizeWeb0195, and B. subtilis Sfp, have been overproduced, purified and found to have P-pant transferase activity, confirming that the observed low level of sequence ... Research Paper … rory mcilroy greWebBelongs to the P-Pant transferase superfamily. Gsp/Sfp/HetI/AcpT family. Curated. Phylogenomic databases. HOGENOM. CLU_119926_0_0_6; InParanoid. P37623; OMA. … rory mcilroy girlfriend tennisWeb31 Jan 2014 · The phosphopantetheinyl transferases (PPTases) are responsible for the activation of the carrier protein domains of the polyketide synthases (PKS), non ribosomal … rory mcilroy golfingWeb20 Mar 2004 · Structure-Based Mutational Analysis of the 4‘-Phosphopantetheinyl Transferases Sfp from Bacillus subtilis: Carrier Protein Recognition and Reaction … rory mcilroy golf pantsWeb1 Jan 2014 · Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. … rory mcilroy highlights today